| Samstag, 06.06.2009 14:00 | |||
| SV Babelsberg 03 - FC Sachsen Leipzig | 1:0 (0:0) |  | |
| Sonntag, 07.06.2009 13:30 | |||
| FC Hansa Rostock II - Hertha BSC II | 3:1 (1:0) | | |
| Hamburger SV II - VfL Wolfsburg II | 2:1 (1:0) | | |
| Hallescher FC - VFC Plauen | 0:1 (0:0) | | |
| Holstein Kiel - VfB Lübeck | 1:0 (0:0) | | |
| Hannover 96 II - SV Wilhelmshaven | 2:1 (0:0) | | |
| Chemnitzer FC - 1. FC Magdeburg | 1:1 (1:0) | | |
| FC Oberneuland - FC Energie Cottbus II | 2:0 (0:0) | | |
| Türkiyemspor 1978 - Altona 93 | 1:0 (0:0) | | |
|
|
| P | Verein | Sp | S | U | N | Diff. | Tore | Pkt. | |
| 1. | (1.) | Holstein Kiel | 34 | 21 | 10 | 3 | 32 | 54:22 | 73 |
| 2. | (2.) | Hallescher FC | 34 | 19 | 13 | 2 | 23 | 43:20 | 70 |
| 3. | (3.) | SV Babelsberg 03 | 34 | 17 | 12 | 5 | 21 | 46:25 | 63 |
| 4. | (4.) | 1. FC Magdeburg | 34 | 17 | 8 | 9 | 14 | 50:36 | 59 |
| 5. | (5.) | VfL Wolfsburg II | 34 | 15 | 8 | 11 | 18 | 53:35 | 53 |
| 6. | (6.) | Hannover 96 II | 34 | 14 | 7 | 13 | 6 | 48:42 | 49 |
| 7. | (8.) | Chemnitzer FC | 34 | 11 | 12 | 11 | 8 | 50:42 | 45 |
| 8. | (7.) | VfB Lübeck | 34 | 10 | 15 | 9 | 4 | 38:34 | 45 |
| 9. | (11.) | FC Oberneuland | 34 | 12 | 8 | 14 | -1 | 40:41 | 44 |
| 10. | (12.) | FC Hansa Rostock II | 34 | 12 | 7 | 15 | -6 | 53:59 | 43 |
| 11. | (9.) | SV Wilhelmshaven | 34 | 12 | 7 | 15 | -7 | 45:52 | 43 |
| 12. | (10.) | Hertha BSC II | 34 | 11 | 10 | 13 | -15 | 40:55 | 43 |
| 13. | (13.) | Hamburger SV II | 34 | 11 | 9 | 14 | 1 | 44:43 | 42 |
| 14. | (14.) | VFC Plauen | 34 | 10 | 12 | 12 | -2 | 52:54 | 42 |
| 15. | (15.) | Türkiyemspor 1978 | 34 | 12 | 6 | 16 | -7 | 36:43 | 42 |
| 16. | (16.) | Altona 93 | 34 | 8 | 7 | 19 | -26 | 40:66 | 31 |
| 17. | (17.) | FC Sachsen Leipzig | 34 | 5 | 9 | 20 | -33 | 21:54 | 24 |
| 18. | (18.) | FC Energie Cottbus II | 34 | 5 | 8 | 21 | -30 | 23:53 | 23 |
